DNA电泳基本参数
分离范围(bp) |
琼脂糖浓度 |
分离范围(bp) |
PAGE浓度 |
1,000~30,000 |
0.5% |
100~1,000 |
3.5% |
800~12,000 |
0.7% |
80~500 |
5.0% |
500~10,000 |
1.0% |
60~400 |
8.0% |
400~7,000 |
1.2% |
40~200 |
12.0% |
200~4,000 |
1.5% |
5~100 |
20.0% |
50~2,000 |
2.0% |
|
|
PAGE浓度 |
(相当核苷酸片段大小,bp) |
|
溴酚蓝 |
二甲苯青 |
|
3.5% |
100 |
460 |
5.0% |
65 |
260 |
8.0% |
45 |
160 |
12.0% |
20 |
70 |
15.0% |
15 |
60 |
20.0% |
12 |
45 |